Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p141 | (1) | WCRB2014

Acquisition of meiotic competence in porcine secondary follicles following long-term culture

Tasaki Hidetaka , Itami Nobuhiko , Iwata Hisataka

The development of culture methods to produce oocytes from immature ovarian follicles in vitro would greatly contribute to livestock production and assisted human reproduction. However, successful in vitro culture to produce fully mature oocytes is very difficult in the case of large domestic animals. In the present study, we investigated the growth properties of in vitro-cultured porcine oocytes derived from early developmental stage follicles. Oocy...

ra0001p146 | (1) | WCRB2014

Resveratrol enhances the in vitro development of oocytes derived from early antral follicles

Itami Nobuhiko , Kuwayama Takehito , Iwata Hisataka

Introduction: The present study aims to investigate the effect of resveratrol supplementation on the in vitro development of porcine oocytes derived from early antral follicles.Materials and methods: Oocyte-granulosa cell complexes (OGCs) were collected from early antral follicles that were 0.5–0.7 mm in diameter and cultured in the absence or present of resveratrol (2 μM) for 14 days. The effect of resveratrol on SIRT1 expression was ...